Essential Ion Channel Methods


Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780123822376
Category: Science
Page: 632
View: 9740
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The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methods Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology

Essential Bioimaging Methods


Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780080963426
Category: Medical
Page: 450
View: 9219
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Need a tested, reliable method that works? Put Essential Bioimaging Methods to work for you. Editor Michael Conn has hand-picked the most robust methods from his previously-published volumes in the Methods in Enzymology series. Many of these methods have been briefly updated by the authors that created them and use them in their research, and this book further refines and organizes existing content and focuses on methods that work, including MRI, fMRI, PET, Microscopic optical imaging and other. Part of the Reliable Lab Solution series, this volume provides clear advice and explicit protocols, providing updates to classic, tried-and-true methods and an essential addition to the bookshelf or workbench of any researcher in the field. * Highlights usefulness of techniques in basic research detailing MRI imaging of small animals, fMRI of Macaque monkeys, and baboon model of reperfused stroke * Built from volumes in the flagship brand, Methods in Enzymology * Provides tricks, tips and different approaches

Handbook of Ion Channels


Author: Jie Zheng,Matthew C. Trudeau
Publisher: CRC Press
ISBN: 1466551429
Category: Medical
Page: 691
View: 1699
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The New Benchmark for Understanding the Latest Developments of Ion Channels Ion channels control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli, and regulate the response to physical stimuli. They can often interact with the cellular environment due to their location at the surface of cells. In nonexcitable tissues, they also help regulate basic salt balance critical for homeostasis. All of these features make ion channels important targets for pharmaceuticals. Handbook of Ion Channels illustrates the fundamental importance of these membrane proteins to human health and disease. Renowned researchers from around the world introduce the technical aspects of ion channel research, provide a modern guide to the properties of major ion channels, and present powerful methods for modeling ion channel diseases and performing clinical trials for ion channel drugs. Conveniently divided into five parts, the handbook first describes the basic concepts of permeation and gating mechanisms, balancing classic theories and the latest developments. The second part covers the principles and practical issues of both traditional and new ion channel techniques and their applications to channel research. The third part organizes the material to follow the superfamilies of ion channels. This part focuses on the classification, properties, gating mechanisms, function, and pharmacology of established and novel channel types. The fourth part addresses ion channel regulation as well as trafficking and distribution. The final part examines several ion channel-related diseases, discussing genetics, mechanisms, and pharmaceutical advances.

Calcium Techniques

A Laboratory Manual
Author: Jan B. Parys,Martin D. Bootman,David I. Yule,Geert Bultynck
Publisher: N.A
ISBN: 9781621820789
Category: Science
Page: 592
View: 3272
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"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--

Molecular Cell Biology


Author: Harvey Lodish
Publisher: Macmillan
ISBN: 9780716776017
Category: Science
Page: 1150
View: 914
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The sixth edition provides an authoritative and comprehensive vision of molecular biology today. It presents developments in cell birth, lineage and death, expanded coverage of signaling systems and of metabolism and movement of lipids.

Sequence — Evolution — Function

Computational Approaches in Comparative Genomics
Author: Eugene Koonin,Michael Y. Galperin
Publisher: Springer Science & Business Media
ISBN: 1475737831
Category: Science
Page: 462
View: 1725
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Sequence - Evolution - Function is an introduction to the computational approaches that play a critical role in the emerging new branch of biology known as functional genomics. The book provides the reader with an understanding of the principles and approaches of functional genomics and of the potential and limitations of computational and experimental approaches to genome analysis. Sequence - Evolution - Function should help bridge the "digital divide" between biologists and computer scientists, allowing biologists to better grasp the peculiarities of the emerging field of Genome Biology and to learn how to benefit from the enormous amount of sequence data available in the public databases. The book is non-technical with respect to the computer methods for genome analysis and discusses these methods from the user's viewpoint, without addressing mathematical and algorithmic details. Prior practical familiarity with the basic methods for sequence analysis is a major advantage, but a reader without such experience will be able to use the book as an introduction to these methods. This book is perfect for introductory level courses in computational methods for comparative and functional genomics.

Laboratory Quality Management System

Handbook
Author: World Health Organization
Publisher: N.A
ISBN: 9789241548274
Category: Medical
Page: 247
View: 6256
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Achieving, maintaining and improving accuracy, timeliness and reliability are major challenges for health laboratories. Countries worldwide committed themselves to build national capacities for the detection of, and response to, public health events of international concern when they decided to engage in the International Health Regulations implementation process. Only sound management of quality in health laboratories will enable countries to produce test results that the international community will trust in cases of international emergency. This handbook was developed through collaboration between the WHO Lyon Office for National Epidemic Preparedness and Response, the United States of America Centers for Disease Control and Prevention (CDC) Division of Laboratory Systems, and the Clinical and Laboratory Standards Institute (CLSI). It is based on training sessions and modules provided by the CDC and WHO in more than 25 countries, and on guidelines for implementation of ISO 15189 in diagnostic laboratories, developed by CLSI. This handbook is intended to provide a comprehensive reference on Laboratory Quality Management System for all stakeholders in health laboratory processes, from management, to administration, to bench-work laboratorians. This handbook covers topics that are essential for quality management of a public health or clinical laboratory. They are based on both ISO 15189 and CLSI GP26-A3 documents. Each topic is discussed in a separate chapter. The chapters follow the framework developed by CLSI and are organized as the "12 Quality System Essentials".

Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book


Author: Richard A. McPherson,Matthew R. Pincus
Publisher: Elsevier Health Sciences
ISBN: 1455726842
Category: Medical
Page: 1568
View: 1904
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Recognized as the definitive book in laboratory medicine since 1908, Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of results. Revisions throughout keep you current on the latest topics in the field, such as biochemical markers of bone metabolism, clinical enzymology, pharmacogenomics, and more! A user-friendly full-color layout puts all the latest, most essential knowledge at your fingertips. Update your understanding of the scientific foundation and clinical application of today's complete range of laboratory tests. Get optimal test results with guidance on error detection, correction, and prevention as well as cost-effective test selection. Reference the information you need quickly and easily thanks to a full-color layout, many new color illustrations and visual aids, and an organization by organ system. Master all the latest approaches in clinical laboratory medicine with new and updated coverage of: the chemical basis for analyte assays and common interferences; lipids and dyslipoproteinemia; markers in the blood for cardiac injury evaluation and related stroke disorders; coagulation testing for antiplatelet drugs such as aspirin and clopidogrel; biochemical markers of bone metabolism; clinical enzymology; hematology and transfusion medicine; medical microbiology; body fluid analysis; and many other rapidly evolving frontiers in the field. Effectively monitor the pace of drug clearing in patients undergoing pharmacogenomic treatments with a new chapter on this groundbreaking new area. Apply the latest best practices in clinical laboratory management with special chapters on organization, work flow, quality control, interpretation of results, informatics, financial management, and establishing a molecular diagnostics laboratory. Confidently prepare for the upcoming recertification exams for clinical pathologists set to begin in 2016.

Ion Channels

A Practical Approach
Author: Richard H. Ashley
Publisher: Oxford University Press
ISBN: 9780199634750
Category: Science
Page: 302
View: 7778
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Research on ion channels now crosses most traditional subject boundaries and involves a wide range of approaches including some of the latest techniques in biochemistry and molecular biology, as well as advanced electrophysiology and biophysics. This volume describes up-to-date practical information for some of the most essential and exciting approaches currently available. Topics covered include patch-clamp recording, concepts of single-channel analysis, channel cloning, channel structure, and signal transduction systems. This will be an invaluable source of practical information for researchers from a variety of academic backgrounds, whose areas of interest might include membrane biology, membrane ion transport, and membrane transport proteins.

Food Analysis


Author: S. Suzanne Nielsen
Publisher: Springer
ISBN: 3319457764
Category: Technology & Engineering
Page: 649
View: 1823
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This fifth edition provides information on techniques needed to analyze foods for chemical and physical properties. The book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information chapters on regulations, labeling, sampling, and data handling provide background information for chapters on specific methods to determine chemical composition and characteristics, physical properties, and objectionable matter and constituents. Methods of analysis covered include information on the basic principles, advantages, limitations, and applications. Sections on spectroscopy and chromatography along with chapters on techniques such as immunoassays, thermal analysis, and microscopy from the perspective of their use in food analysis have been expanded. Instructors who adopt the textbook can contact the editor for access to a website with related teaching materials.

Schmidek and Sweet: Operative Neurosurgical Techniques E-Book

Indications, Methods and Results (Expert Consult - Online and Print)
Author: Alfredo Quinones-Hinojosa
Publisher: Elsevier Health Sciences
ISBN: 1455723282
Category: Medical
Page: 2592
View: 6472
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Wherever, whenever, or however you need it, unmatched procedural guidance is at your fingertips with the new edition of Schmidek & Sweet: Operative Neurosurgical Techniques! Completely revised under the auspices of new editor-chief Dr. Alfredo Quiñones-Hinojosa, this comprehensive medical reference examines indications, operative techniques, complications, and results for nearly every neurosurgical procedure. Full-color illustrations, 21 new chapters, internationally-acclaimed contributors, surgical videos, and online access make it a "must have" for today's practitioner. Hone your skills for Master virtually every routine and specialized procedure for brain, spinal, and peripheral nerve problems in adult patients. Review clinical information on image-guided technologies and infections. Easily understand and apply techniques with guidance from more than 1,600 full-color illustrations. Rely on the knowledge and experience of new editor-in-chief Dr. Alfredo Quiñones-Hinojosa and leading international authorities, who offer multiple perspectives on neurosurgical challenges, from tried-and-true methods to the most current techniques. See exactly how to proceed with online surgical videos that guide you through each technique and procedure to ensure the best possible outcomes and results. Apply the latest techniques and knowledge in deep brain stimulation for epilepsy, movement disorders, dystonia, and psychiatric disorders; surgical management of blast injuries; invasive electrophysiology in functional neurosurgery; and interventional management of cerebral aneurysms and arterio-venous malformations. Take it with you anywhere! Access the full text, downloadable image library, video clips, and more at www.expertconsult.com.

Fundamentals of Electric Propulsion

Ion and Hall Thrusters
Author: Dan M. Goebel,Ira Katz
Publisher: John Wiley & Sons
ISBN: 9780470436264
Category: Technology & Engineering
Page: 486
View: 8012
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Throughout most of the twentieth century, electric propulsion was considered the technology of the future. Now, the future has arrived. This important new book explains the fundamentals of electric propulsion for spacecraft and describes in detail the physics and characteristics of the two major electric thrusters in use today, ion and Hall thrusters. The authors provide an introduction to plasma physics in order to allow readers to understand the models and derivations used in determining electric thruster performance. They then go on to present detailed explanations of: Thruster principles Ion thruster plasma generators and accelerator grids Hollow cathodes Hall thrusters Ion and Hall thruster plumes Flight ion and Hall thrusters Based largely on research and development performed at the Jet Propulsion Laboratory (JPL) and complemented with scores of tables, figures, homework problems, and references, Fundamentals of Electric Propulsion: Ion and Hall Thrusters is an indispensable textbook for advanced undergraduate and graduate students who are preparing to enter the aerospace industry. It also serves as an equally valuable resource for professional engineers already at work in the field.

ADME-Enabling Technologies in Drug Design and Development


Author: Donglu Zhang,Sekhar Surapaneni
Publisher: John Wiley & Sons
ISBN: 0470542780
Category: Medical
Page: 591
View: 661
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This book comprehensively covers the state-of-the-art and cutting-edge technologies in an integrated fashion for applications in ADME studies of small molecular drugs. Each chapter provides descriptions of the technologies, application scope and limitations, optimal conditions for intended results, protocols, case studies, and future developments. By concisely describing these technologies, the book provides a useful tool for drug metabolism scientists and as a reference for scientists in the fields of pharmacology, medicinal chemistry, pharmaceutics, toxicology, bioanalytical science in academia and industry.

Physiology of Excitable Membranes

Proceedings of the 28th International Congress of Physiological Sciences, Budapest, 1980
Author: J. Salánki,H. Meves,N. Chalazonitis
Publisher: Elsevier
ISBN: 1483146928
Category: Science
Page: 370
View: 1759
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Physiology of Excitable Membranes contains plenary lecture and most of the papers presented at five symposia of the Section ""General Cell Physiology"" at the 28th International Congress of Physiological Sciences. Organized into 44 chapters, this book begins with a discussion on the ionic mechanisms of excitability of nerve cells. Subsequent chapters focus on charge movement in nerve membrane; calcium electrogenesis; optical changes during electrogenesis; synaptic transmission and modulation; and transmission in autonomic ganglia.

Ion channels of vascular smooth muscle cells and endothelial cells

proceedings of the International Society for Heart Research (ISHR) held in Cincinnati, Ohio, May 28 through June 2, 1991
Author: International Society for Heart Research. Meeting,Hiroshi Kuriyama
Publisher: Elsevier Science Ltd
ISBN: N.A
Category: Science
Page: 374
View: 1203
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Ion Channels as Therapeutic Targets


Author: N.A
Publisher: Academic Press
ISBN: 0128052104
Category: Science
Page: 402
View: 2823
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Ion Channels as Therapeutic Targets is the latest volume in the popular Advances in Protein Chemistry and Structural Biology series, an essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins, with each thematically organized volume guest edited by leading experts in a broad range of protein-related topics. Provides cutting-edge developments in protein chemistry and structural biology Discusses the use of ion channels as therapeutic targets Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students

Handbook of Models for Human Aging


Author: P. Michael Conn
Publisher: Elsevier
ISBN: 9780080460062
Category: Medical
Page: 1075
View: 1857
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The Handbook of Models for Human Aging is designed as the only comprehensive work available that covers the diversity of aging models currently available. For each animal model, it presents key aspects of biology, nutrition, factors affecting life span, methods of age determination, use in research, and disadvantages/advantes of use. Chapters on comparative models take a broad sweep of age-related diseases, from Alzheimer's to joint disease, cataracts, cancer, and obesity. In addition, there is an historical overview and discussion of model availability, key methods, and ethical issues. Utilizes a multidisciplinary approach Shows tricks and approaches not available in primary publications First volume of its kind to combine both methods of study for human aging and animal models Over 200 illustrations

Fundamental Neuroscience


Author: Larry R. Squire
Publisher: Academic Press
ISBN: 0123858704
Category: Medical
Page: 1127
View: 3735
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Fundamental Neuroscience, 3rd Edition introduces graduate and upper-level undergraduate students to the full range of contemporary neuroscience. Addressing instructor and student feedback on the previous edition, all of the chapters are rewritten to make this book more concise and student-friendly than ever before. Each chapter is once again heavily illustrated and provides clinical boxes describing experiments, disorders, and methodological approaches and concepts. A companion web site contains test questions, and an imagebank of the figures for ready use in presentations, slides, and handouts. Capturing the promise and excitement of this fast-moving field, Fundamental Neuroscience, 3rd Edition is the text that students will be able to reference throughout their neuroscience careers! New to this edition: * 30% new material including new chapters on Dendritic Development and Spine Morphogenesis, Chemical Senses, Cerebellum, Eye Movements, Circadian Timing, Sleep and Dreaming, and Consciousness * Companion website with figures, web links to additional material, and test questions * Additional text boxes describing key experiments, disorders, methods, and concepts * Multiple model system coverage beyond rats, mice, and monkeys * Extensively expanded index for easier referencing

Steam, Water, and Hydrothermal Systems

Physics and Chemistry Meeting the Needs of Industry : Proceedings of the 13th International Conference on the Properties of Water and Steam
Author: Peter R. Tremaine,International Association for the Properties of Water and Steam
Publisher: NRC Research Press
ISBN: 0660177781
Category: Feed-water
Page: 954
View: 1952
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This work includes 140 papers on pure and applied research of physics and chemistry of hydrothermal systems. It includes papers on metastable states, nucleation, super-cooled water and high temperature aqueous solutions.

American Biotechnology Laboratory


Author: N.A
Publisher: N.A
ISBN: N.A
Category: Biology
Page: N.A
View: 1625
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