Essential Ion Channel Methods

Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780123822376
Category: Science
Page: 632
View: 4014
The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methods Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology

Pumps, Channels and Transporters

Methods of Functional Analysis
Author: Ronald J. Clarke,Mohammed A. A. Khalid
Publisher: John Wiley & Sons
ISBN: 1119085136
Category: Science
Page: 488
View: 6500
Describes experimental methods for investigating the function of pumps, channels and transporters Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions

Biological Membrane Ion Channels

Dynamics, Structure, and Applications
Author: Shin-Ho Chung,Olaf S. Anderson,Vikram V. Krishnamurthy
Publisher: Springer Science & Business Media
ISBN: 0387689192
Category: Science
Page: 658
View: 6037
This book deals with recent breakthroughs in ion-channel research that have been brought about by the combined effort of experimental biophysicists and computational physicists, who together are beginning to unravel the story of these exquisitely designed biomolecules. With chapters by leading experts, the book is aimed at researchers in nanodevices and biosensors, as well as advanced undergraduate and graduate students in biology and the physical sciences.

Ion Channels

Methods and Protocols
Author: James D. Stockand,Mark S. Shapiro
Publisher: Springer Science & Business Media
ISBN: 1597450952
Category: Science
Page: 212
View: 1286
Ion Channels is about practical implementation of the study of ion channels and should be used as a field guide by the investigator when designing and performing research on channels. Each chapter provides detail about a particular aspect of investigating ion channels. The detail includes description of the actual successfully employed experimental procedures and pitfalls for each application.

Ion Channels

Author: Bernardo Rudy,Linda E. Iverson
Publisher: Gulf Professional Publishing
ISBN: 9780126016956
Category: Science
Page: 917
View: 1021
This is an unabridged, comb bound version of Ion Channels: Volume 207 of Methods of Enzymology. Techniques for the electrophysiological recording of ion channels in a number of different cell types, including microorganisms, are described in this volume. Key Features * Applications of electrophysiological techniques * Expression in heterologous systems * Reconstitution of lipid bilayers * Purification and characterization of ion channel proteins and genes * Recording of ion channels of intracellular membranes and microorganisms * Data storage and analysis

Ion Channels and Disease

Author: Frances M. Ashcroft
Publisher: Academic Press
ISBN: 0080535216
Category: Science
Page: 481
View: 8636
Ion channels are membrane proteins that act as gated pathways for the movement of ions across cell membranes. They play essential roles in the physiology of all cells. In recent years, an ever-increasing number of human and animal diseases have been found to result from defects in ion channel function. Most of these diseases arise from mutations in the genes encoding ion channel proteins, and they are now referred to as the channelopathies. Ion Channels and Disease provides an informative and up-to-date account of our present understanding of ion channels and the molecular basis of ion channel diseases. It includes a basic introduction to the relevant aspects of molecular biology and biophysics and a brief description of the principal methods used to study channelopathies. For each channel, the relationship between its molecular structure and its functional properties is discussed and ways in which genetic mutations produce the disease phenotype are considered. This book is intended for research workers and clinicians, as well as graduates and advanced undergraduates. The text is clear and lively and assumes little knowledge, yet it takes the reader to frontiers of what is currently known about this most exciting and medically important area of physiology. Key Features * Introduces the relevant aspects of molecular biology and biophysics * Describes the principal methods used to study channelopathies * Considers single classes of ion channels with summaries of the physiological role, subunit composition, molecular structure and chromosomal location, plus the relationship between channel structure and function * Looks at those diseases associated with defective channel structures and regulation, including mutations affecting channel function and to what extent this change in channel function can account for the clinical phenotype

Ion Channel Reconstitution

Author: C. Miller
Publisher: Springer Science & Business Media
ISBN: 9780306421365
Category: Science
Page: 577
View: 5483

Methods in Neuronal Modeling

From Ions to Networks
Author: Christof Koch,Idan Segev
Publisher: MIT Press
ISBN: 9780262112314
Category: Medical
Page: 671
View: 2524
This book serves as a handbook of computational methods and techniques for modeling the functional properties of single and groups of nerve cells.

Ion channel screening: advances in technologies and analysis

Author: N.A
Publisher: Frontiers E-books
ISBN: 2889190358
Page: N.A
View: 8162

Cholesterol Regulation of Ion Channels and Receptors

Author: Irena Levitan,Francisco Barrantes
Publisher: John Wiley & Sons
ISBN: 1118342305
Category: Science
Page: 302
View: 4747
Examines new research on the role of cholesterol inregulating ion channels and receptors and its effect onhealth Drawing together and analyzing all the latest research findings,this book explores the role of cholesterol in the regulation of ionchannels and receptors, including its pathological effects. It isthe first book to comprehensively describe the complex mechanismsby which cholesterol regulates two major classes of membraneproteins. Moreover, it sheds new light on how cholesterol affectsessential cellular functions such as the contraction of the heart,propagation of nerve impulses, and regulation of blood pressure andkidney function. Written and edited by leading pioneers in the field,Cholesterol Regulation of Ion Channels and Receptors isdivided into three parts: Part I, Cholesterol Regulation of Membrane Properties,introduces the heterogeneity of cholesterol distribution inbiological membranes and the physical and biological implicationsof the formation of cholesterol-rich membrane domains. Part II, Cholesterol Regulation of Ion Channels,examines the mechanisms underlying cholesterol sensitivities of ionchannels, including the regulation of ion channels by cholesterolas a boundary lipid. Part III, Cholesterol Regulation of Receptors, exploresthe latest discoveries concerning how cholesterol regulatesdistinct types of receptors, including G-protein coupled receptors,LDL and scavenger receptors, and innate immune systemreceptors. Increased levels of cholesterol represent a major health risk.Understanding cholesterol regulation of ion channels and receptorsis essential for facilitating the development of new therapeuticstrategies to alleviate the impact of pathological cholesterolconditions. With this book as their guide, readers have access tothe most current knowledge in the field.

Der lange Weg zur Freiheit

Author: Nelson Mandela
Publisher: S. Fischer Verlag
ISBN: 3104031541
Category: History
Page: 864
View: 1741
»Ich bin einer von ungezählten Millionen, die durch Nelson Mandelas Leben inspiriert wurden.« Barack Obama Eine fast drei Jahrzehnte währende Gefängnishaft ließ Nelson Mandela zum Mythos der schwarzen Befreiungsbewegung werden. Kaum ein anderer Politiker unserer Zeit symbolisiert heute in solchem Maße die Friedenshoffnungen der Menschheit und den Gedanken der Aussöhnung aller Rassen wie der ehemalige südafrikanische Präsident und Friedensnobelpreisträger. Auch nach seinem Tod finden seine ungebrochene Charakterstärke und Menschenfreundlichkeit die Bewunderung aller friedenswilligen Menschen auf der Welt. Mandelas Lebensgeschichte ist über die politische Bedeutung hinaus ein spannend zu lesendes, kenntnis- und faktenreiches Dokument menschlicher Entwicklung unter Bedingungen und Fährnissen, vor denen die meisten Menschen innerlich wie äußerlich kapituliert haben dürften.

Receptor and Ion Channel Detection in the Brain

Methods and Protocols
Author: Rafael Luján,Francisco Ciruela
Publisher: Humana Press
ISBN: 9781493930630
Category: Medical
Page: 475
View: 1070
Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

The Neuron

Cell and Molecular Biology
Author: Irwin B. Levitan,Leonard K. Kaczmarek
Publisher: Oxford University Press, USA
ISBN: 9780195145236
Category: Medical
Page: 603
View: 632
The Neuron provides an up-to-date comprehensive first course in the biology of nerve cells. It explains how the latest breakthroughs in genomic mapping and imaging techniques have illuminated the mechanisms by which a neuron generates varied patterns of electrical activity and transfers this information to other neurons. It also covers the molecular factors that shape the development of the mature nervous system and allow it to generate phenomena such as learning and memory.


Author: Jon Cooper,Tony Cass
Publisher: Oxford University Press, USA
ISBN: 9780191538698
Category: Science
Page: 268
View: 7919
Over the past 20 years, biosensors have revolutionised the care and management of diabetes and have had important impacts in several other areas of clinical diagnostics. This new book is a completely revised edition of Biosensors - A Practical Approach published in 1990. Edited by two internationally renowned experts in this field, it draws together contributions from active researchers in Europe, North America and Asia. Chapters explain how to implement diverse techniques, such as protein engineering, optical and electrochemical instrumentation and numerical modelling, in the context of producing biosensors for both laboratory and commercial applications. The book offers an overview of current research in this area as well as pointers to its further directions. It will be suitable both for those already active in the area who wish to expand their repertoire of experimental tools and for those who are just starting out in biosensor research. - ;Over the past 20 years, the field of biosensor research have had a significant impact in both laboratory research and the commercial sector. Over that period, biosensors have revolutionised the care and management of diabetes and have had important impacts in several other areas of clinical diagnostics. Europe, North America and Asia-Pacific have all seen the rise of small and medium sized companies seeking technical and application niches in the manufacture or use of biosensors. The current activity in both gene and protein 'biochips' can be seen as the latest set of tools that allow users who are not analytical science practitioners to make technically complex and reliable measurements with the minimum of intervention. Similarly the concern about the dissemination of chemical or biological weapons and the need for their rapid and reliable detection will need to be met by devices that have many characteristics in common with biosensors. This book provides a practical introduction to the many skills needed in the highly interdisciplinary field of biosensor technology. Edited by two internationally renowned experts in this field it draws together contributions from active researchers in Europe, North America and Asia who describe how to implement techniques as diverse as protein engineering, optical and electrochemical instrumentation, single cell electrophysiology, screen printing and numerical modelling in the context of producing biosensors for both laboratory and commercial applications. As well as the many detailed protocols and experimental tips, the book also offers an overview of current research in this area as well as pointers to its further directions. The diversity of topics covered means that it will be suitable both for those already active in the area who wish to expand their repertoire of experimental tools and for those who are just starting out in biosensors research. - ;... written by well-known researchers in the field. A strength of the book is that it features topical technologies, such as redox hydrogels, oligonucleotide sensitive electrodes, ion channels and protein engineering, but has not forgotten what has made biosensors the commercial success they are today ... easy to read, but provides sufficient depth to keep experienced researchers interested. - Chemistry & Industry

Cell-based Biosensors

Principles and Applications
Author: Qingjun Liu,Ping Wang
Publisher: Artech House
ISBN: 1596934409
Category: Biosensors
Page: 271
View: 6760
Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.

Essentials of Neural Science and Behavior

Author: Eric R. Kandel,James Harris Schwartz,Thomas M. Jessell
Publisher: McGraw Hill Professional
ISBN: 9780838522455
Category: Medical
Page: 743
View: 5863
This textbook presents the fundamental principles of neuroscience and its effect on behavior. Neuroscience is the scientific study of the nervous system. Topics will include: principles of brain organization; structure and ultrastructure of neurons; neurophysiology and biophysics of excitable cells; synaptic transmission; neurotransmitter systems and neurochemistry; molecular biology of neurons; development and plasticity of the brain; aging and diseases of the nervous system; organization of sensory and motor systems; structure and function of cerebral cortex; modeling of neural systems. It also examines such topics as mammalian sensory, motor, regulatory, and motivational mechanisms involved in the control of behavior, and higher mental processes such as those involved in language and memory.

Cellular Neurobiology

A Practical Approach
Author: John Chad,H. V. Wheal
Publisher: Oxford University Press
ISBN: 9780199631070
Category: Cells, Cultured.
Page: 287
View: 8343
Cellular Neurobiology brings together a large number of techniques which are useful in exploring the functions of molecules, and the resolution of neurobiological processes at the cellular level.


Pharmacological and Clinical Profile of a Calcium Antagonist
Author: M. Kaltenbach
Publisher: Springer-Verlag
ISBN: 3662073641
Category: Medical
Page: 144
View: 3078

Developmental Psychopathology, Theory and Methods

Author: Dante Cicchetti,Donald J. Cohen
Publisher: Wiley
ISBN: 9780471532439
Category: Psychology
Page: 816
View: 4238
The first of two complementary volumes on developmental psychopathology, a new perspective on mental illness that ties mental disorder to normal development. Developmental psychopathology is sufficiently different from older views of mental dysfunction as to be growing as a separate subdiscipline of psychology/psychiatry. This volume covers the history, theory and methods of this new approach, including the contributions of several standard theoretical viewpoints (psychoanalytic, system, theory, organizational, information processing, cross-cultural, etc.); examines the relationship between developmental psychopathology and genetics, neuropsychology, epidemiology, and ethology; and looks at the implications for psychometric theory.

Handbook of Imaging in Biological Mechanics

Author: Corey P. Neu,Guy M. Genin
Publisher: CRC Press
ISBN: 1466588144
Category: Medical
Page: 579
View: 3486
Emerging imaging techniques have opened new fronts to investigate tissues, cells, and proteins. Transformative technologies such as microCT scans, super-resolution microscopy, fluorescence-based tools, and other methods now allow us to study the mechanics of cancer, dissect the origins of cellular force regulation, and examine biological specimens