Comprehensive Enzyme Kinetics

This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics.

Author: Vladimir Leskovac

Publisher: Springer Science & Business Media

ISBN: 9780306483905

Category: Science

Page: 438

View: 924

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Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.
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Enzyme Kinetics and Mechanism

Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data.

Author: Paul F. Cook

Publisher: Garland Science

ISBN: 9781136844287

Category: Medical

Page: 416

View: 934

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Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.
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Enzyme Kinetics

This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.

Author: Hans Bisswanger

Publisher: John Wiley & Sons

ISBN: 9783527622030

Category: Science

Page: 320

View: 668

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This new, expanded and updated edition of the user-friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.
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Enzyme Kinetics Catalysis and Control

Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, ...

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 9780123809254

Category: Science

Page: 920

View: 973

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Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions
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A Study of Enzymes

This particular volume provides important information for both the novice and the seasoned investigator.

Author: Stephen A. Kuby

Publisher: CRC Press

ISBN: 0849369878

Category: Medical

Page: 488

View: 508

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This comprehensive monograph consists of two parts: Volume I, entitled Enzyme Catalysis, Kinetics, and Substrate Binding; and Volume II, entitled Mechanism of Enzyme Action. Volume I focuses on several aspects of enzyme catalytic behavior, their steady-state and transient-state kinetics, and the thermodynamic properties of substrate binding. Packed with figures, tables, schemes, and photographs, this volume contains over 1,000 references, including references regarding enzymology's fascinating history. This comprehensive book is of particular interest to enzymology students, teachers, and researchers. Volume II presents selected "cutting edge" examples of techniques and approaches being pursued in biochemistry. This up-to-date resource includes 11 chapters, which illustrate important theoretical and practical aspects of enzyme mechanisms. It also features selected examples in which today's most important techniques, ideas, and theories are used to elaborate on the intricate nature of enzyme action mechanisms. This particular volume provides important information for both the novice and the seasoned investigator.
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Enzyme Kinetics

This 1977 book covers in a comprehensive manner the detailed kinetic analysis of a number of different enzyme models.

Author: D. V. Roberts

Publisher: CUP Archive

ISBN: 052121274X

Category: Science

Page: 326

View: 989

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This 1977 book covers in a comprehensive manner the detailed kinetic analysis of a number of different enzyme models.
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Initial Rate Enzyme Kinetics

A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University.

Author: H. J. Fromm

Publisher: Springer Science & Business Media

ISBN: 9783642809668

Category: Science

Page: 324

View: 515

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Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.
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Handbook of Biochemical Kinetics

The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms.

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 0080521932

Category: Science

Page: 788

View: 430

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Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes. Key Features * Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes * Over 1,500 definitions of kinetic and mechanistic terminology, with key references * Practical advice on experimental design of kinetic experiments * Extended step-by-step methods for deriving rate equations * Over 1,000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods * Over 5,000 selected references to kinetic methods appearing in the Methods in Enzymology series * 72-page Wordfinder that allows the reader to search by keywords * Summaries of mechanistic studies on key enzymes and protein systems * Over 250 diagrams, figures, tables, and structures
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Enzyme Mechanism from Isotope Effects

Enzyme Mechanism from Isotope Effects is an excellent reference source for investigators using isotope effects in their research. The book is also valuable for reference libraries and instructors teaching courses in enzyme mechanism.

Author: Paul F. Cook

Publisher: CRC Press

ISBN: 0849353122

Category: Medical

Page: 432

View: 470

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Isotope effects have become one of the most powerful tools available to the enzymologist for probing enzymic mechanisms. Enzyme Mechanism from Isotope Effects presents the basic theory underlying isotope effects, including the latest findings on proton tunneling and coupled atomic notions. Specific theoretical applications are emphasized in regard to the types of information that can be obtained using isotope effects. The book also examines recent theoretical treatments of the product dependence of deuterium isotope effects, multiple isotope effects and isotope effects on intermediate partitioning. Other topics include a complete discussion of methods for measuring isotope effects, including a detailed description of the use of the isotope ratio mass spectrometer to obtain isotope effects, and a review of the literature regarding mechanistic information obtained from isotope effects for individual classes of enzyme-catalyzed reactions. Enzyme Mechanism from Isotope Effects is an excellent reference source for investigators using isotope effects in their research. The book is also valuable for reference libraries and instructors teaching courses in enzyme mechanism.
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Metabolic Engineering

Detailed kinetic modeling of metabolism has been hampered by the requirement
for extensive and explicit enzyme kinetics . In general , complete enzyme kinetic
information is not available for the entire metabolism of a single cell . The human
 ...

Author: Sang Yup Lee

Publisher: CRC Press

ISBN: 082477390X

Category: Science

Page: 450

View: 142

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This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.
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Enzymology Labfax

Information is also provided on, for example, coenzymes and analogs, and the analysis of ligand binding and metalloenzyme reactivity, as well as important information on buffers, chelating agents and detergents required on a daily basis.The ...

Author: Paul C. Engel

Publisher: Academic Press

ISBN: STANFORD:36105018327671

Category: Clinical enzymology

Page: 289

View: 554

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LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. The authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs. Enzymology Labfax is a comprehensive compendium of data on enzyme kinetics, measurement of enzyme activity, physical factors which affect activity and patterns of enzyme inhibition. Information is also provided on, for example, coenzymes and analogs, and the analysis of ligand binding and metalloenzyme reactivity, as well as important information on buffers, chelating agents and detergents required on a daily basis.The contents of Enzymology Labfax have been carefully chosen to complement the data presented in Proteins Labfax , which is therefore the companion volume to this text.
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Enzyme Reactor Engineering

This book begins with an organized introduction to enzyme reactor engineering, followed by two major parts—analysis of enzyme reaction kinetics, and analysis of enzyme reactor features.

Author: F. Xavier Malcata

Publisher: Wiley

ISBN: 1119998964

Category: Technology & Engineering

Page: 1008

View: 295

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Provides a thorough study of the engineering of enzyme reactors, including comprehensive mathematical modeling and optimization Enzyme Reactor Engineering: Principles and Applications sequentially covers the three classical levels of description: macroscopic, or ideal; microscopic, or nonideal in terms of hydrodynamics (including homogeneous, nontrivial flow patterns, as well as heterogeneous systems); and submicroscopic, in terms of mixing. Major emphasis is placed on general simulation from first principles, rather than empirical correlation. This methodology rationally departs from balance equations, carefully eliminates overparameterization, and establishes dimensionless, simpler relationships; and builds on such models to find optima of relevance, while constructing rational strategies to approach common problems. This book begins with an organized introduction to enzyme reactor engineering, followed by two major parts—analysis of enzyme reaction kinetics, and analysis of enzyme reactor features. It concludes with a brief coverage of relevant mathematical concepts. A carefully paced approach, suitable even for nonspecialists, allows the reader to gain insight about the detailed kinetics of the reaction brought about by a general enzyme, and provides the complementary tools necessary to design and optimize the overall reactor behavior. Provides thorough study of the engineering of enzyme reactors, including comprehensive mathematical modeling, and coverage of additional topics (e.g. separation, control) required for effective integration and overall understanding Chapters introduce basic phenomenological principles and subsequently derive usable results, ending up with generic examples of germane applications Environmental concerns supporting white biotechnology, and a growing portfolio of available, tailored and less expensive enzymes on the market have turned enzyme reactors into a better and better performing (and recommended) technology for industrial implementation. Enzyme Reactor Engineering is thus the ideal text to support that effort–suitable for students, researchers, and practitioners working in chemical engineering, biochemistry, biological engineering, chemistry, physical chemistry, and applied physics.
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Comprehensive Biomaterials

Enzyme–substrate interaction Enzymes are highly specific and efficient in their
reaction with substrates. They act ... This results in a pseudo-first-order kinetic of
the reaction with regard to the concentration of the enzyme according to eqn [10]
 ...

Author: Paul Ducheyne

Publisher: Elsevier

ISBN: 9780080552941

Category: Technology & Engineering

Page: 3672

View: 786

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Comprehensive Biomaterials brings together the myriad facets of biomaterials into one, major series of six edited volumes that would cover the field of biomaterials in a major, extensive fashion: Volume 1: Metallic, Ceramic and Polymeric Biomaterials Volume 2: Biologically Inspired and Biomolecular Materials Volume 3: Methods of Analysis Volume 4: Biocompatibility, Surface Engineering, and Delivery Of Drugs, Genes and Other Molecules Volume 5: Tissue and Organ Engineering Volume 6: Biomaterials and Clinical Use Experts from around the world in hundreds of related biomaterials areas have contributed to this publication, resulting in a continuum of rich information appropriate for many audiences. The work addresses the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, and strategic insights for those entering and operational in diverse biomaterials applications, research and development, regulatory management, and commercial aspects. From the outset, the goal was to review materials in the context of medical devices and tissue properties, biocompatibility and surface analysis, tissue engineering and controlled release. It was also the intent both, to focus on material properties from the perspectives of therapeutic and diagnostic use, and to address questions relevant to state-of-the-art research endeavors. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance as well as future prospects Presents appropriate analytical methods and testing procedures in addition to potential device applications Provides strategic insights for those working on diverse application areas such as R&D, regulatory management, and commercial development
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Enzyme Assays

A discussion on the analysis of kinetic data is included in Chapter 10 of this book
and the reader is referred to several comprehensive accounts for detailed
treatments of the steady - state kinetics of enzyme - catalysed reactions ( 4 - 7 , 48
- 51 ) ...

Author: Robert Eisenthal

Publisher: Practical Approach (Paperback)

ISBN: 0199638209

Category: Medical

Page: 282

View: 293

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Principles of enzyme assay and kinetic studies; Photometric assays; Radiometric assays; High performance liquid chromatographic assays; Electrochemical assays: the oxygen electrode; Electrochemical assays: the nitric oxide electrode; Electrochemical assays: the ph-stat; Enzyme assays after gel electrophoresis; Techniques for enzyme extraction; Determination of active site concentration; High throughput screening - considerations for enzyme assays; Statistical analysis of enzyme kinetic data.
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Enzyme Kinetics and Mechanism Part F Detection and Characterization of Enzyme Reaction Intermediates

S. Waldo and E. C. Theil, in “Comprehensive Supramolecular Chemistry” (K. S.
Suslick, ed.), p. 65. Elsevier ... rights reserved. 0076-6879/02 $35.00 5 D. M.
Lawson, P. J. Artymiuk, S. J. Yewdall, 436 ENZYME KINETICS AND
MECHANISM [32]

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 0080489389

Category: Science

Page: 650

View: 975

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates
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Comprehensive chemical kinetics

Classical models of enzyme kinetics As has already been pointed out, any rate
equation containing the concentration of the free catalyst is of little practical use if
that concentration is unknown, is difficult or impossible to measure, and may vary
 ...

Author: C. H. Bamford

Publisher:

ISBN: CHI:69667772

Category: Chemical kinetics

Page:

View: 387

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Analysis of Enzyme Reaction Kinetics 2 Volume Set

The second volume introduces readers to physicochemical modulation of reaction rate – starting with basic concepts, and looking specifically at temperature-, mechanical force-, pH- and compound-driven effects: both unimodal and bimodal ...

Author: F. Xavier Malcata

Publisher: Wiley

ISBN: 1119490243

Category: Technology & Engineering

Page: 1472

View: 264

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Comprehensively introduces readers to modelling of rate of enzymatic reactions, including effects of physicochemical parameters Analysis of Enzyme Reaction Kinetics is the second set in a unique eleven-volume collection on Enzyme Reactor Engineering. It describes rate expressions pertaining to enzymatic reactions, including modulation by physicochemical factors, as well as tools for prediction and control of how fast substrates are transformed to products. Volume 1 details rate expressions mathematically derived from mechanistic postulates, and is complemented by appropriate statistical approaches to fit them to experimental data. Volume 2 discusses the effects of physical and chemical parameters upon the rates of both enzyme-catalyzed and enzyme-deactivation reactions. Starting with basic concepts and historical perspectives, the first volume introduces readers to the mathematics of rate expressions. It then goes on to cover kinetic features and the many forms of Michaelis & Menten’s-type rate expressions (single and multiple enzymes, autocatalysis, single and multiple substrates, multiphasic systems, etc.), and concludes with the statistical analysis of rate expressions – including the assessment of data, fitting of models to data, and generation of data themselves. The second volume introduces readers to physicochemical modulation of reaction rate – starting with basic concepts, and looking specifically at temperature-, mechanical force-, pH- and compound-driven effects: both unimodal and bimodal deactivation are considered. Analysis of Enzyme Reaction Kinetics 2V Set is a comprehensive work for those studying or working with enzyme reactions, or practitioners involved in the control of reactors. SERIES INFORMATION Enzyme Reactor Engineering is organized into four major sets: Enzyme Reaction Kinetics and Reactor Performance; Analysis of Enzyme Reaction Kinetics; Analysis of Enzyme Reactor Performance; and Mathematics for Enzyme Reaction Kinetics and Reaction Performance.
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Comprehensive Biotechnology

It follows , therefore , that an immobilized enzyme typically shows apparently
enzyme - independent kinetics at low concentrations but normal
enzymedependent kinetics at high concentrations . From the practical point of
view this means that ...

Author: Murray Moo-Young

Publisher: World Bank Publications

ISBN: 0080325092

Category: Biotechnology

Page: 688

View: 207

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Biotechnology is defined as the evaluation and use of biological agents and materials in the production of goods and services for industry, trade and commerce. In this four-volume set there are two main divisions of the subject matter: an academic coverage of the disciplinary underpinnings of the field in Volumes 1 and 2, followed by a practical view of the various processes and products in Volumes 3 and 4. In the integration of these two areas, other common factors dealing with product quality, process economics and government policies are introduced at appropriate points throughout all four volumes. Volume 1 specifically delineates and integrates the unifying multidisciplinary principles in terms of relevant genetic, biological, chemical and biochemical fundamentals. As in the other volumes, a glossary of terms and nomenclature guidelines is included to assist both the beginner and the non-specialist.
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New Comprehensive Biochemistry

CHAPTER 3 Enzyme kinetics PAUL C . ENGEL Department of Biochemistry ,
University of Sheffield , Sheffield S10 2TN , Great Britain ime 1 . Introduction :
aims and approaches in enzyme kinetics Enzyme - catalysed reactions occur as
a ...

Author:

Publisher:

ISBN: CHI:22383653

Category: Biochemistry

Page:

View: 914

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