Critical Microscopy

This is a reproduction of the original artefact. Generally these books are created from careful scans of the original. This allows us to preserve the book accurately and present it in the way the author intended.

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ISBN: 0371389712

Category: History

Page: 128

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Critical Microscopy

About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work.

Author: Alfred Charles Coles

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ISBN: 0282589422

Category: Photography

Page: 122

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Excerpt from Critical Microscopy: How to Get the Best Out of the MicroscopeIt does not attempt to describe the various models of the microscope or their accessories, all of which can be seen in the makers' catalogues, and, therefore, illustrations of them are omitted.About the PublisherForgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.comThis book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
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Critical Microscopy How to Get the Best Out of the Microscope

We are republishing these classic works in affordable, high quality, modern editions, using the original text and artwork.

Author: Alfred Charles Coles

Publisher: Blatter Press

ISBN: 1446088235

Category: Literary Collections

Page: 114

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Many of the earliest books, particularly those dating back to the 1900s and before, are now extremely scarce and increasingly expensive. We are republishing these classic works in affordable, high quality, modern editions, using the original text and artwork.
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Superresolution Optical Microscopy

This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes.

Author: Barry R. Masters

Publisher: Springer Nature

ISBN: 9783030216917

Category: Science

Page: 403

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This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
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Critical Microscopy How to Get the Most Out of the Microscope 1922

This scarce antiquarian book is a facsimile reprint of the original.

Author: Alfred Charles Coles

Publisher: Kessinger Publishing

ISBN: 1104047314

Category: Literary Collections

Page: 116

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This scarce antiquarian book is a facsimile reprint of the original. Due to its age, it may contain imperfections such as marks, notations, marginalia and flawed pages. Because we believe this work is culturally important, we have made it available as part of our commitment for protecting, preserving, and promoting the world's literature in affordable, high quality, modern editions that are true to the original work.
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A Practical Guide to Optical Microscopy

If in our case n i > n twhen θ i exceeds a critical angle then total internal reflection occurs. This angle is defined by sin θ c = n tn i or more normally θ c = sin − 1 (n tn i) The critical angle clearly depends on the ratio of the ...

Author: John Girkin

Publisher: CRC Press

ISBN: 9781351630351

Category: Science

Page: 260

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Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book
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Cantor Lectures on the Microscope

One ambitious form of microscope has been 5. ... conveniently used the student is to be advised to train his hand as condensers , they would add much to the and eye in the critical estimation of optical interest of popular microscopy .

Author: John Mayall

Publisher:

ISBN: UCBK:C005976082

Category: Microscopes

Page: 97

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An Introduction to Microscopy by Means of Light Electrons X Rays or Ultrasound

Number 1% cover glasses are specified by ASTM to have thicknesses of 0.17–0.19 mm.” The variation is permissible in the interest of economy. For “criticalmicroscopy the cover glasses should be 0.180 + 0.002 mm thick unless the ...

Author: Eugene Rochow

Publisher: Springer Science & Business Media

ISBN: 9781468424546

Category: Technology & Engineering

Page: 367

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Many people look upon a microscope as a mere instrument(l); to them microscopy is instrumentation. Other people consider a microscope to be simply an aid to the eye; to them microscopy is primarily an expan sion of macroscopy. In actuality, microscopy is both objective and sub jective; it is seeing through an instrument by means of the eye, and more importantly, the brain. The function of the brain is to interpret the eye's image in terms of the object's structure. Thought and experience are required to distinguish structure from artifact. It is said that Galileo (1564-1642) had his associates first look through his telescope microscope at very familiar objects to convince them that the image was a true representation of the object. Then he would have them proceed to hitherto unknown worlds too far or too small to be seen with the un aided eye. Since Galileo's time, light microscopes have been improved so much that performance is now very close to theoretical limits. Electron microscopes have been developed in the last four decades to exhibit thousands of times the resolving power of the light microscope. Through the news media everyone is made aware of the marvelous microscopical accomplishments in imagery. However, little or no hint is given as to what parts of the image are derived from the specimen itself and what parts are from the instrumentation, to say nothing of the changes made during preparation of the specimen.
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