Electron Tomography

This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series.

Author: Joachim Frank

Publisher: Springer Science & Business Media

ISBN: 9780387690087

Category: Science

Page: 456

View: 802

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This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
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Electron Tomography

This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.

Author: Joachim Frank

Publisher: Springer Science & Business Media

ISBN: 9781475721638

Category: Science

Page: 399

View: 948

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This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.
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Cellular Imaging

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques.

Author: Eric Hanssen

Publisher: Springer

ISBN: 9783319689975

Category: Science

Page: 323

View: 455

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This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
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Cellular Electron Microscopy

Each section of the book is preceded by an introduction, which should help newcomers understand the subject.

Author: J. Richard McIntosh

Publisher: Elsevier

ISBN: 0080475035

Category: Science

Page: 880

View: 783

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Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going
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Transmission Electron Microscopy

This text is a companion volume to Transmission Electron Microscopy: A Textbook for Materials Science by Williams and Carter.

Author: C. Barry Carter

Publisher: Springer

ISBN: 9783319266510

Category: Technology & Engineering

Page: 518

View: 423

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This text is a companion volume to Transmission Electron Microscopy: A Textbook for Materials Science by Williams and Carter. The aim is to extend the discussion of certain topics that are either rapidly changing at this time or that would benefit from more detailed discussion than space allowed in the primary text. World-renowned researchers have contributed chapters in their area of expertise, and the editors have carefully prepared these chapters to provide a uniform tone and treatment for this exciting material. The book features an unparalleled collection of color figures showcasing the quality and variety of chemical data that can be obtained from today’s instruments, as well as key pitfalls to avoid. As with the previous TEM text, each chapter contains two sets of questions, one for self assessment and a second more suitable for homework assignments. Throughout the book, the style follows that of Williams & Carter even when the subject matter becomes challenging—the aim is always to make the topic understandable by first-year graduate students and others who are working in the field of Materials Science Topics covered include sources, in-situ experiments, electron diffraction, Digital Micrograph, waves and holography, focal-series reconstruction and direct methods, STEM and tomography, energy-filtered TEM (EFTEM) imaging, and spectrum imaging. The range and depth of material makes this companion volume essential reading for the budding microscopist and a key reference for practicing researchers using these and related techniques.
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Introduction to Electron Microscopy for Biologists

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level.

Author:

Publisher: Academic Press

ISBN: 9780080888163

Category: Science

Page: 560

View: 958

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This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). *Basic techniques in transmission and scanning electron microscopy *Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton *Discussion on electron microscopy of viruses and virus-cell interactions
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Transmission Electron Microscopy and Diffractometry of Materials

This book explains concepts of transmission electron microscopy (TEM) and x-ray diffractometry (XRD) that are important for the characterization of materials.

Author: Brent Fultz

Publisher: Springer Science & Business Media

ISBN: 9783642297618

Category: Science

Page: 764

View: 260

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This book explains concepts of transmission electron microscopy (TEM) and x-ray diffractometry (XRD) that are important for the characterization of materials. The fourth edition adds important new techniques of TEM such as electron tomography, nanobeam diffraction, and geometric phase analysis. A new chapter on neutron scattering completes the trio of x-ray, electron and neutron diffraction. All chapters were updated and revised for clarity. The book explains the fundamentals of how waves and wavefunctions interact with atoms in solids, and the similarities and differences of using x-rays, electrons, or neutrons for diffraction measurements. Diffraction effects of crystalline order, defects, and disorder in materials are explained in detail. Both practical and theoretical issues are covered. The book can be used in an introductory-level or advanced-level course, since sections are identified by difficulty. Each chapter includes a set of problems to illustrate principles, and the extensive Appendix includes laboratory exercises.
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Electron Microscopy of Model Systems

The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology.

Author:

Publisher: Academic Press

ISBN: 0123810086

Category: Science

Page: 744

View: 456

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The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. Covers the preparation and analysis of model systems for biological electron microscopy Includes the most popular systems but also organisms that are less frequently used in cell biology Presents the currently most important methods for the preparation of biological specimens First compendium covering the various aspects of sample preparation of very diverse biological systems
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Three Dimensional Electron Microscopy

Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow ...

Author:

Publisher: Academic Press

ISBN: 9780128170199

Category: Science

Page: 303

View: 321

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Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the Three-Dimensional Electron Microscopy technique
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Scanning Transmission Electron Microscopy of Nanomaterials

This volume covers recent achievements in the field of STEM obtained with advanced technologies such as spherical aberration correction, monochromator, high-sensitivity electron energy loss spectroscopy and the software of image mapping.

Author: Nobuo Tanaka

Publisher: World Scientific

ISBN: 9781783264711

Category: Science

Page: 616

View: 231

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The basics, present status and future prospects of high-resolution scanning transmission electron microscopy (STEM) are described in the form of a textbook for advanced undergraduates and graduate students. This volume covers recent achievements in the field of STEM obtained with advanced technologies such as spherical aberration correction, monochromator, high-sensitivity electron energy loss spectroscopy and the software of image mapping. The future prospects chapter also deals with z-slice imaging and confocal STEM for 3D analysis of nanostructured materials. Contents:Introduction (N Tanaka)Historical Survey of the Development of STEM Instruments (N Tanaka)Basic Knowledge of STEM:Basics of STEM (N Tanaka and K Saitoh)Application of STEM to Nanomaterials and Biological Specimens (N Shibata, S D Findlay, Y Ikuhara and N Tanaka)Theories of STEM Imaging:Theory for HAADF-STEM and Its Image Simulation (K Watanabe)Theory for Annular Bright Field STEM Imaging (S D Findlay, N Shibata and Y Ikuhara)Electron Energy-Loss Spectroscopy in STEM and Its Imaging (K Kimoto)Density Functional Theory for ELNES in STEM-EELS (T Mizoguchi)Advanced Methods in STEM:Aberration Correction in STEM (H Sawada)Secondary Electron Microscopy in STEM (H Inada and Y Zhu)Scanning Confocal Electron Microscopy (K Mitsuishi and M Takeguchi)Electron Tomography in STEM (N Tanaka)Electron Holography and Lorentz Electron Microscopy in STEM (N Tanaka)Recent Topics and Future Prospects in STEM (N Tanaka) Readership: Graduate students and researchers in the field of nanomaterials and nanostructures. Key Features:Most advanced; befitting beginning graduate studentsVery convenient for advanced researchers who would like to use STEM and have a comprehensive understanding of the theory of image contrast and application detailsSpans from the basic theory to the applications of STEMKeywords:STEM;Nanomaterials;HAADF-STEM;Atomic Resolution;Elemental Mapping;Dark Field Images;Nanoanalysis;Nanofabrication;NanodiffractionReviews: “This is written in a very readable style, packed with information and helpful explanations, and above all, very up to date. The book is generously illustrated, with many nice line-drawings, historic photographs, micrographs and spectra and, as a bonus, it has a name index as well as a subject index.” Ultramicroscopy
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Experimental Atomic Resolution Electron Tomography

This work focus on the efforts made to obtain three-dimensional local information at atomic resolution using electron tomography.

Author: Mary Cooper Scott

Publisher:

ISBN: OCLC:1078232729

Category:

Page: 132

View: 848

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This work focus on the efforts made to obtain three-dimensional local information at atomic resolution using electron tomography. To realize this long-standing goal, state-of-the art experimental and computational methods were developed. This thesis describes the experimental portion of the collaborative project that has resulted in the first true atomic resolution reconstruction of a material while making no assumptions of crystallinity. Chapter 1 will give background information on electron microscopy and tomography. Chapter 2 describes the experimental aspects of atomic scale electron tomography using an uncorrected electron microscope. Chapters 3 and 4 follow from this development, and describe the results obtained from implementing this methodology. By reconstructing the data obtained with these methods with an advanced algorithm, atomic scale features were observed for the first time in three dimensions. As a first demonstration, a gold nanoparticle was reconstructed with 2.4 angstrom resolution, revealing the internal twin boundaries and grain structure of a multiply twinned icosahedral nanoparticle. Next, improvements in data and Bragg filtering revealed the three dimensional atomic structure of edge and screw dislocations, as well as stacking faults, for the first time. Chapter 5 then discusses the additional experimental considerations needed to employ an aberration corrected electron microscope for the tomographic data. Also in this chapter, a novel needle geometry for tomography is introduced. Chapters 6 and 7 then discuss the results of this methodology applied to both needle and conventional samples. Here, true atomic resolution is finally achieved, with atomic positions being precisely located within the sample, and a point defect identified. With the 3D atomic positions determined, displacement and strain fields can be calculated at high resolution. Work is underway to achieve not only atomic resolution, but also to differentiate between atomic species within the sample. Electron tomography is a powerful technique for characterization in three dimensions, and this work represents the highest resolution yet achieved, and the first demonstration of true atomic resolution electron tomography. The ability to localize the three-dimensional positions of atoms in a material while making no assumptions of crystallinity, as well as measure point defects and strain, is an important breakthrough and will find broad application in many fields.
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Biological Electron Microscopy

The TOM core complex : The general protein import pore of the outer membrane of mitochondria . J. Cell Biol . 147 : 959 . Burmeister , W. , Grimm , R. , and Walz , J. 1999. Electron tomography of molecules and cells . Trends Cell Biol .

Author: Michael J. Dykstra

Publisher: Springer Science & Business Media

ISBN: 0306477491

Category: Science

Page: 534

View: 771

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Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
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Correlative Light and Electron Microscopy

This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing ...

Author: Thomas Müller-Reichert

Publisher: Academic Press

ISBN: 9780124160262

Category: Science

Page: 404

View: 105

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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron micros© CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
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Transmission Electron Microscopy

This fourth edition includes discussion of recent progress, especially in the area of Schottky emission guns, convergent-beam electron diffraction, electron tomography, holography and the high resolution of crystal lattices.

Author: Ludwig Reimer

Publisher: Springer

ISBN: 9783662148242

Category: Science

Page: 587

View: 883

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Transmission Electron Microscopy presents the theory of image and contrast formation, and the analytical modes in transmission electron microscopy. The principles of particle and wave optics of electrons are described. Electron-specimen interactions are discussed for evaluating the theory of scattering and phase contrast. Also discussed are the kinematic and dynamical theories of electron diffraction and their applications for crystal-structure analysis and imaging of lattices and their defects. X-ray micronanalysis and electron energy-loss spectroscopy are treated as analytical methods. This fourth edition includes discussions of recent progress, especially in the area of Schottky emission guns, convergent-beam electron diffraction, electron tomography, holography and the high resolution of crystal lattices.
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Correlative Light and Electron Microscopy II

This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM).

Author:

Publisher: Academic Press

ISBN: 9780128013212

Category: Science

Page: 452

View: 835

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This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)
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Biological Electron Microscopy

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines.

Author: Michael J. Dykstra

Publisher: Springer Science & Business Media

ISBN: 9781441992444

Category: Science

Page: 534

View: 896

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Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
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Electron Tomography

This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series.

Author: Joachim Frank

Publisher: Springer

ISBN: 0387511741

Category: Science

Page: 456

View: 509

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This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
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Biological Field Emission Scanning Electron Microscopy

Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM ...

Author: Roland A. Fleck

Publisher: John Wiley & Sons

ISBN: 9781118663264

Category: Science

Page: 752

View: 277

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The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
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Aberration Corrected Analytical Transmission Electron Microscopy

However, in microscopy in general, there is increasing interest in the determination of three dimensional information—known as tomography. Electron tomography using both CTEM and STEM can be achieved by recording (usually) images for a ...

Author: Rik Brydson

Publisher: John Wiley & Sons

ISBN: 9781119979906

Category: Science

Page: 296

View: 702

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The book is concerned with the theory, background, and practical use of transmission electron microscopes with lens correctors that can correct the effects of spherical aberration. The book also covers a comparison with aberration correction in the TEM and applications of analytical aberration corrected STEM in materials science and biology. This book is essential for microscopists involved in nanoscale and materials microanalysis especially those using scanning transmission electron microscopy, and related analytical techniques such as electron diffraction x-ray spectrometry (EDXS) and electron energy loss spectroscopy (EELS).
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