Endometrial Gene Expression

This book presents the state of the art in endometrial gene expression as well as recent developmental findings relating to reproduction and reproductive disorders.

Author: Joanne Kwak-Kim

Publisher: Springer Nature

ISBN: 9783030285845

Category: Health & Fitness

Page: 220

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The endometrium goes through cyclical changes under the influence of hormones, and immune effectors in the endometrium change their populations cyclically as well. Dynamic changes in immune effectors determine endometrial cytokine and chemokine milieus and, consequently, the endometrial immune response. Endometrial immune responses have been shown to be important for trophoblast invasion and early pregnancy. Indeed, dysregulated endometrial inflammatory immune responses are associated with reproductive failures, such as recurrent pregnancy losses, repeated implantation failure, and gynecological diseases with oncogenic potential. Hence, the investigation of endometrial gene expression may predict infertility, gynecological and oncological conditions. This book presents the state of the art in endometrial gene expression as well as recent developmental findings relating to reproduction and reproductive disorders. It begins with a review of the genetic regulation of urogenital tract formation and molecular mechanism underlying the physiology of menstrual cycle before moving on to the current and emerging technological advances in molecular biology. Endometrial gene expressions of immune inflammatory conditions, viral infection, metabolic and nutritional conditions, and reproductive disorders are then presented in following chapters. Examples of the use and interpretation of gene expression in clinical scenarios including recurrent pregnancy losses, infertility and multiple implantation failures are presented with currently available endometrial gene analysis. Utilizing the latest evidence and clinical guidelines, Endometrial Gene Expression will be a cutting-edge resource for gynecologist, reproductive immunologist, reproductive endocrinologists, obstetrician, maternal fetal medicine specialist and other health care providers in the field of reproductive medicine.
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Role of Ulipristal Acetate in Regulating Endometrial Gene Expression and Spheroids Attachment

All rights not granted by the above license are retained by the author. Abstract: The novel emergency contraceptive Ulipristal acetate (UPA) belongs to the progesterone receptor modulator family.

Author: Yingxing Li

Publisher: Open Dissertation Press

ISBN: 1361380578

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This dissertation, "Role of Ulipristal Acetate in Regulating Endometrial Gene Expression and Spheroids Attachment" by Yingxing, Li, 李莹星, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: The novel emergency contraceptive Ulipristal acetate (UPA) belongs to the progesterone receptor modulator family. A single oral dosage of 30mg UPA within 120 hours of unprotected intercourse could delay ovulation and differentiation of endometrium. Yet, whether UPA affect embryo implantation remains largely unknown. This study aims to investigate whether UPA affect endometrial gene expressions and embryo attachment onto endometrial epithelial cells. The PR-expressing human endometrial carcinoma cell line Ishikawa was used and treated with 10nM estrogen, 1μM progesterone or 4μM UPA for 24 hours. Changes in transcriptome profiles were analyzed by Affymetrix Human Gene 1.0 ST array GeneChip. Gene clustering showed the gene expression pattern after UPA treatment was similar to control (0.1% ethanol); while estrogen treated group was different from all the other groups. Totally, 8 genes were significantly increased and 1 was decreased (>=2-fold, pThe effect of UPA on human embryo-endometrium attachment was carried out using an in vitro multi-cellular spheroids-endometrial epithelial cell co-culture model. Human choriocarcinoma cell line JAR and Ishikawa were used. UPA (0.04-4μM) treatment for up to 48 hours did not affect the proliferation of JAR or Ishikawa cells. Similarly, the attachment of JAR spheroids onto Ishikawa cells after 1 hour co-culture was not affected by UPA treatment. The molecules of Wnt/β-catenin signaling pathway, a pathway that is actively involved in embryo implantation, such as the β-catenin and GSK-3β, and endometrial receptive marker E-cadherin were not changed after UPA treatment. In Ishikawa cells, the expression of PR-A was induced after UPA (0.04-4μM) treatment; while PR-B increased when 0.04 or 4μM UPA used. However, the PR-A/PR-B ratio remained unchanged after all concentration of UPA treatment. The effect of UPA on spheroids attachment was further investigated with cultured human primary endometrial epithelial cells. Endometrial glandular epithelial cells were digested and isolated from endometrial biopsy taken from IVF patients on day 7 after luteinizing hormone surge (LH+7). A co-culture assay was optimized with JAR spheroids and endometrial epithelial cells that were growing on Matrigel. The attachment rate of JAR spheroids is approximately 60% after 3 hours incubation. However, after 24 hours of exposure to 4μM UPA, the attachment remained comparable to that of the control group. In conclusion, UPA could alter the expression of genes in Ishikawa cells mainly related to angiogenesis. It is likely that UPA may affect stromal decidualization and blastocyst invasion after attachment. However, UPA did not affect the expression of Wnt-signaling molecules and attachment of JAR spheroids onto either Ishikawa or human primary endometrial epithelial cell. DOI: 10.5353/th_b5060565 Subjects: Human embryo Gene expression Contraceptives Endometrium
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Progesterone Regulation of Endometrial Gene Expression in the Early Pregnant Ovine Uterus

Establishment of pregnancy in ruminants requires blastocyst development to form an elongated filamentous conceptus that produces interferon tau (IFNT), the pregnancy recognition signal, and initiate implantation.

Author: Megan A. Minten

Publisher:

ISBN: OCLC:818248139

Category:

Page:

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Establishment of pregnancy in ruminants requires blastocyst development to form an elongated filamentous conceptus that produces interferon tau (IFNT), the pregnancy recognition signal, and initiate implantation. Blastocyst growth and development is dependent upon secretions from the uterine endometrium. An early increase in post-ovulatory circulating levels of progesterone (P4) stimulates blastocyst growth and conceptus elongation in ruminants. Microarray analysis was used to identify candidate P4-regulated genes and regulatory networks in the endometrium that govern peri-implantation blastocyst/conceptus growth and development. The first study was conducted to validate effects of P4 and/or pregnancy on expression of candidate genes identified by microarray analysis. The genes included: ANGPTL3, CHGA, CLEC4E, CXCL14, EFNA1, EFNB1, FABP3, IFNG, IL6, LGALS3, PTH, RBP4, SLIT2, SLIT3, and VWF. Early P4 treatment up-regulated CXCL14 gene expression in Day 9 ovine endometrium compared to control endometrium, and FABP3, IFNG, IL6 and LGALS3 in Day 12 early P4-treated ovine endometrium. Expression of ANGPTL3, CHGA, CXCL14, EFNA1, EFNB1, LGALS3 and RBP4 was affected by day of pregnancy. Treatment of ewes with P4+RU486 (P4 receptor antagonist) reduced expression of ANGPTL3, CHGA, EFNA1, EFNB1, FABP3, IFNG, IL6, LGALS3, RBP4, and SLIT2, SLIT3 and VWF in comparison to Day 12 P4-treated endometrium. The second study evaluated expression of genes identified by microarray analysis in endometrium from pregnant and cyclic ewes. Genes evaluated included those from the first study. ANGPTL3, CHGA, CXCL14, EFNA1, EFNB1, IFNG, LGALS3, PTH, RBP4, SLIT2, SLIT3 and VWF were affected by day, status and/or their interaction between Days 10 and 16. Of note, FABP3 increased 21-fold between Days 14 to 18 of pregnancy, and IL6 increased 37-fold between Days 14 to 20 of pregnancy. In situ hybridization analysis detected FABP3 mRNA in both luminal and superficial glandular epithelia of pregnant ewes and trophectoderm, whereas IL6 mRNA was detected in immune cells within uterine luminal epithelium and glandular epithelium and trophectoderm. Collectively, these results identify candidate genes encoding for biologically active molecules that regulate growth and development of the ovine conceptus during the peri-implantation period of pregnancy.
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Human Endometrial Gene Expression Profiling and Receptivity in Patients Undergoing in Vitro Fertilization Ivf Treatment

This dissertation, "Human Endometrial Gene Expression Profiling and Receptivity in Patients Undergoing in Vitro Fertilization (IVF) Treatment" by Yunao, Liu, 劉蘊奡, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and ...

Author: Yunao Liu

Publisher: Open Dissertation Press

ISBN: 1374704768

Category:

Page:

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This dissertation, "Human Endometrial Gene Expression Profiling and Receptivity in Patients Undergoing in Vitro Fertilization (IVF) Treatment" by Yunao, Liu, 劉蘊奡, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b4308540 Subjects: Steroid hormones Estradiol Endometrium Gene expression Ovulation - Induction Fertilization in vitro, Human
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Changes in Endometrial Gene Expression During the Peri implantation Period in the Rat microform

GRP78, a molecular chaperone residing in the endoplasmic reticulum, was identified by mRNA differential display as having an expression pattern unique to the maximally sensitized endometrium.

Author: David Simmons

Publisher: National Library of Canada = Bibliothèque nationale du Canada

ISBN: 0612681084

Category:

Page: 362

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GRP78, a molecular chaperone residing in the endoplasmic reticulum, was identified by mRNA differential display as having an expression pattern unique to the maximally sensitized endometrium. Specifically, an induction of Grp78 mRNA and protein within the uterine glandular epithelium of the maximally sensitized/receptive endometrium is reported. The hormonal regulation of Grp78 by P4 and E2 appears to be indirect as maximal induction of Grp78 mRNA requires 48 h of progesterone priming followed by exposure to estrogen, the same hormonal requirements for the onset of receptivity/sensitization. (Abstract shortened by UMI.).
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Transcriptomic Approach to Study the Effect of Polypectomy on Human Endometrial Receptivity

This dissertation, "Transcriptomic Approach to Study the Effect of Polypectomy on Human Endometrial Receptivity" by Kin-wa, Chan, 陳建華, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to ...

Author: Kin-Wa Chan

Publisher: Open Dissertation Press

ISBN: 1361352051

Category:

Page:

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This dissertation, "Transcriptomic Approach to Study the Effect of Polypectomy on Human Endometrial Receptivity" by Kin-wa, Chan, 陳建華, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Background: Endometrial polyps are common in women at reproductive ages, and found in more than 11% of infertile women. Hysteroscopic polypectomy is recommended to alleviating symptoms in patients with abnormal uterine bleeding. While the relationship between an endometrial polyp and subfertility is not clearly established, there is some evidence that hysteroscopic polypectomy may improve fertility outcome in IVF patients. Accumulating evidence suggested that elevated levels of activated mast cells as well as elevated matrix MMPs and cytokines levels in the patients who suffer from endometrial polyps. Moreover, significant increases in glycodelin levels were found in patients' plasma and uterine. And there was a significant negative relationship between plasma glycodelin and progesterone levels in patients with endometrial polyps. To further understand whether hysteroscopic polypectomy affects endometrial microenvironment that is conductive to embryo implantation, we recruited 9 IVF patients and use microarray to compare their endometrial gene expressions before and after polypectomy. Methods: We investigated the change of endometrial gene expression patterns before and after polypectomy. Three out of 9 samples were subjected to microarray study. Quantitative-PCR (qPCR) was used to confirm the expression in all 9 samples, and compared with endometrial samples taken from patients underwent natural (LH+7 days, n = 8) and stimulated (hCG+7 days, n = 8) IVF cycles but without polyps. The outcome of subsequent IVF cycle was obtained to correlate the change of gene expression patterns. Results: Microarray analysis demonstrated that no genes are commonly differentially expressed in all 3 samples. One of the samples has shown very heterogeneous result. Sixteen genes (hERα, hERβ, OLFM1, OLFM2, IL15, CXCL14, GPX3, CCL4, UPKIB, PAEP, MMP26, SLC1A1, MUC15, MUC16, TM4SF4 and TFPI2) that are differentially expressed in any 2 of the samples and genes related to endometrial receptivity (OLFM1, IL15, CXCL14, GPX3, PAEP, MMP26, SLC1A1, MUC16) were selected for further analysis. We found that 3 samples have a gene expression pattern similar to natural cycle (LH+7 days) after polypectomy; while the other samples have a gene expression similar to the stimulated cycle (hCG+7 days). Clinical findings of the treated patients did not have strong correlation with pregnancy outcome. One out of two patients who had gene expression patterns similar to natural cycle got pregnant; while one out of seven patients who has gene expression patterns similar to stimulated cycle get pregnant after polypectomy. Conclusion: Polypectomy modulates endometrial gene expression patterns in some patients similar to those in natural cycles. However, our data did not support endometrial polypectomy significantly improved pregnancy outcome in the limited number of patient subjects (n=9) in this study. DOI: 10.5353/th_b5318882 Subjects: Uterus - Endoscopic surgery
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