Genetic Manipulation

PROBLEMS OF GENETIC ENGINEERING IN ANIMAL BREEDING G. F.
Stranzinger Institute for Animal Production, Animal Breeding Section, ETH
Zuerich Zentrum, CH-8092 Zuerich, Switzerland ABSTRACT Remarks are given
in respect to ...

Author: Werner Arber

Publisher: Cambridge University Press

ISBN: 0521264170

Category: Science

Page: 250

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Twenty-three papers by the Committee on Genetic Experimentation (COGENE) review recent advances in experimental studies on microorganisms, plants and animals.
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Testing for Genetic Manipulation in Plants

Following the rational development of genetic engineering, many genetically
modified (GM) crops are now being exploited in the world. However, the safety of
GM crops regarding public health and the environment has not been thoroughly ...

Author: John Flex Jackson

Publisher: Springer Science & Business Media

ISBN: 3540431535

Category: Science

Page: 194

View: 696

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The debate over genetic manipulation and its use in plant improvement and protection has led to an increased demand for developing methods for detecting and characterizing genetic manipulation in plants and plant products such as seeds and foods. This book is unique in presenting all relevant methods together in one volume: those for using and determining markers retained in genetically manipulated products as well as methods for eliminating marker genes and procedures for characterizing chromosomal aberrations in genetically manipulated plants.
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Genetic Manipulation of the Nervous System

Hence the in vivo delivery of genes which promoted the ability to regenerate
might allow improved recovery following such injury. Similarly, since the genes
encoding the heat shock proteins (hsps) have been shown to protect neuronal
cells ...

Author:

Publisher: Elsevier

ISBN: 0080532403

Category: Science

Page: 276

View: 242

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Neuroscience Perspectives provides multidisciplinary reviews of topics in one of the most diverse and rapidly advancing fields in the life sciences. Whether you are a new recruit to neuroscience, or an established expert, look to this series for 'one-stop' sources of the historical, physiological, pharmacological, biochemical, molecular biological and therapeutic aspects of chosen research areas. The recent development of Gene Therapy procedures which allow specific genes to be delivered to human patients who lack functional copies of them is of major therapeutic importance. In addition such gene delivery methods can be used in other organisms to define the function of particular genes. These studies are of particular interest in the nervous system where there are many incurable diseases like Alzheimer's and Parkinson's diseases which may benefit from therapies of this kind. Unfortunately gene delivery methods for use in the nervous system have lagged behind those in other systems due to the fact that the methods developed in other systems are often not applicable to cells like neurons which do not divide. This book discusses a wide range of methods which have now been developed to overcome these problems and allow safe and efficient delivery of particular genes to the brain. Methods discussed include virological methods, physical methods (such as liposomes) and the transplantation of genetically modified cells. In a single volume therefore this book provides a complete view of these methods and indicates how they can be applied to the development of therapies for treating previously incurable neurological disorders.
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Genetic Manipulation

These procedures are collectively termed genetic manipulation or, more
popularly, genetic engineering. Genetic manipulation is no longer the province
ofthe specialized researcher. The techniques are now finding widespread
application in ...

Author: J. M. Grange

Publisher: John Wiley & Sons

ISBN: 9781444314137

Category: Science

Page: 416

View: 637

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Genetic manipulation is no longer the province of the specialized researcher. It is finding widespread application in all fields of medicine and biology. Nevertheless, application of these relatively new techniques to new areas of research is often fraught with unexpected problems and difficulties. Based on the Society for Applied Bacteriology's Autumn 1989 Conference, this unique volume covers a wide and very up-to-date range of techniques used in genetic engineering. These include the isolation and analysis of DNA and RNA from cells and tissues, the selection and use of phage and plasmic vectors for cloning DNA, the cloning procedures, the production and screening of genomic libraries, the production and use of DNA probes, the polymerase chain reaction and the synthesis of 'designer' genes. This volume contains many examples of the applications of the above and other techniques for genetic manipulation, to subjects as diverse as plant pathology, forensic science, bacterial taxonomy, cardiac research, diagnostic microbiology, food hygiene and sewage treatment.
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Genetic Manipulation in Crops

Proceedings of the International Symposium on Genetic Manipulation in Crops,
the 3rd International Symposium on Haploidy, the 1st International Symposium
on Somatic Cell Genetics in Crops, Beijing, October 1984 International Rice ...

Author:

Publisher: Int. Rice Res. Inst.

ISBN: 9781851480227

Category: Crops

Page: 446

View: 445

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Special lectures; Haploid; Mutation, Mutagenesis; Somaclonal variation; Somatic morphogenesis; Protoplasts; Molecular biology of plant gene and genetic manipulation.
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The Methodology of Plant Genetic Manipulation Criteria for Decision Making

Proceedings of the Eucarpia Plant Genetic Manipulation Section Meeting held at
Cork, Ireland from September 11 to September 14, 1994 Alan C. Cassells, Peter
Jones. Public perception and the release of transgenic plants Several surveys of
 ...

Author: Alan C. Cassells

Publisher: Springer Science & Business Media

ISBN: 9789401103572

Category: Science

Page: 477

View: 456

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A range of novel techniques is available to the plant breeder today to complement classical breeding methods. The new options are based on the integration of advances in plant cell biology with those in plant molecular biology. Plant cell, tissue and organ cultures provide efficient systems for transformation, for the achievement of wide crosses and for the production of variation through spontaneous and induced mutation, while permitting effective isolation of desired genotypes by in vitro selection. This book presents a critical appraisal of the methodologies of plant genetic manipulation for advanced undergraduates, postgraduates, researchers and plant breeders, and provides guidance on the choice of breeding options. The latter depends on the breeding system of the crop, the breeding objective and the tissue culture systems applicable to the target genotype(s).
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Testicular Function From Gene Expression to Genetic Manipulation

Mol Hum Reprod 3:699–704 Vereb M, Agulnik AI, Houston JT, Lipschultz LI,
LambDJ, BishopCE(1997) Absenceof DAZ gene mutations in case of
nonobstructed azoospermia. Mol Hum Reprod 3:55–59 Vogt P,Chandley AC,
Hargreave TB, ...

Author: Mario Stefanini

Publisher: Springer Science & Business Media

ISBN: 9783662036716

Category: Medical

Page: 314

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This volume contains the text of the plenary lectures given by internationally recognized experts in the field of gametogenesis and endocrinology at the 10th European Workshop on Molecular and Cellular Endocrinology of the Testis. Topics include spermatogonial transplantation, germ stem cell biology, regulation of meiosis and spermiogenesis, growth factors, cell signaling and transduction, steriodogenesis, androgen-regulated genes, sperm activation and sperm factors active at fertilization. The present volume pays particular attention to the use of genetically manipulated animal models in the study of the regulation of spermatogenesis and testicular functions.
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Genetic Manipulation

Author: North Atlantic Assembly

Publisher:

ISBN: OCLC:7831385

Category: Genetic regulation

Page: 60

View: 962

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Genetic Modification in the Food Industry

Horror movies dote on terrifying images of nature subverted . Secondly and more
generally , the whole image of genetic engineering can generate strong
emotional and moral reactions . Many surveys have revealed public concern
about the ...

Author: Susan Harlander

Publisher: Springer Science & Business Media

ISBN: 0751403997

Category: Science

Page: 260

View: 775

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Intended for food industry professionals with little or no background in biotechnology, this book provides an introduction to the basic concepts of gene modification in the food industry.
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Principles of Gene Manipulation

An Introduction to Genetic Engineering R. W. Old, S. B. Primrose. Chapter 8 .
Expression of Cloned DNA Molecules Following the demonstration ( see p . 33 )
that a gene ( App ) originating from Staphylococcus aureus can function in an ...

Author: R. W. Old

Publisher: Univ of California Press

ISBN: 0520046269

Category: Genetic engineering

Page: 214

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An Introduction to Genetic Engineering

The diagnosis and treatment of human disease is one area in which genetic
manipulation is beginning to have a considerable effect. As outlined in Chapter
10, many therapeutic proteins are now made by recombinant DNA methods, and
the ...

Author: Desmond S. T. Nicholl

Publisher: Cambridge University Press

ISBN: 0521004713

Category: Science

Page: 292

View: 416

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The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
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Principles of Gene Manipulation and Genomics

Sandy B. Primrose, Richard Twyman. Appendix: The genetic code and
singleletter amino acid designations Second position U Ser C A G c a v. Leu Arg
A U ...

Author: Sandy B. Primrose

Publisher: John Wiley & Sons

ISBN: 9781118653883

Category: Science

Page: 672

View: 573

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The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics. Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics Includes two new chapters on the applications of genomics An accompanying website - www.blackwellpublishing.com/primrose - provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format. An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.
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Manipulation

An over-emphasis on direct genetic manipulation, or unrealistic hopes for it, can
have a negative impact on man's primary responsibility to promote genuinely
human evolution and development through better attitudes, better behaviour and
a ...

Author: Bernhard Häring

Publisher:

ISBN: STANFORD:36105036423569

Category: Bioethics

Page: 218

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The Ethics of Inheritable Genetic Modification

... future technology or a future society , it is still meant to be seen as a
contribution to the assessment of the powers and dangers of genetic
manipulation of human and non - human bodies and to the decisions to be
undertaken in the present .

Author: John Rasko

Publisher: Cambridge University Press

ISBN: 0521822777

Category: Medical

Page: 315

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Is inheritable genetic modification the new dividing line in gene therapy? The editors of this searching investigation, representing clinical medicine, public health and biomedical ethics, have established a distinguished team of scientists and scholars to address the issues from the perspectives of biological and social science, law and ethics, including an intriguing Foreword from Peter Singer. Their purpose is to consider how society might deal with the ethical concerns raised by inheritable genetic modification, and to re-examine prevailing views about whether these procedures will ever be ethically and socially justifiable. The book also provides background to define the field, and discusses the biological and technological potential for inheritable genetic modification, its limitations, and its connection with gene therapy, cloning, and other reproductive interventions. For scientists, bioethicists, clinicians, counsellors and public commentators, this is an essential contribution to one of the critical debates in current genetics.
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Molecular Genetic Modification of Eucaryotes

The interest has been motivated by the need to expand genetic accessability of
desirable characteristics of plants and ... to investigate somatic hybridization and
other forms of genetic manipulation in higher plant cells (Gamborg and Wetter, ...

Author: Irwin Rubenstein

Publisher: Academic Press

ISBN: 9781483272351

Category: Science

Page: 186

View: 950

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Molecular Genetic Modification of Eucaryotes reviews the current state of knowledge and techniques potentially useful for the molecular genetic modification of eucaryotes. The book interfaces molecular genetics, plant cell and tissue culture, and plant improvement. Topics include cell and tissue culture, protoplasts, somatic cell fusion, cellular mutagenesis, regeneration of new plant types, and the applicability of these techniques to plant improvements. Viruses and viral integration; integration and expression of foreign genetic material in human cells, Drosophila, plant cells, and legumes; biophysical studies of DNA uptake by plants; and genetic engineering for plant protection against diseases are also discussed in detail. The book will be of interest to geneticists, cell biologists, plant breeders, plant physiologists, plant pathologists, and biochemists.
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Gene Cloning and Manipulation

A number of Gram-negative bacteria are also of particular interest for genetic
manipulation. They include Agrobacterium rhizogenes and Agrobacterium
tumefaciens, plant pathogens of special importance for the genetic manipulation
of plants ...

Author: Christopher Howe

Publisher: Cambridge University Press

ISBN: 0521407001

Category: Medical

Page: 210

View: 966

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"Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduate, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations."--BOOK JACKET.
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The Genetic Manipulation of Staphylococci

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can ...

Author: Jeffrey L. Bose

Publisher:

ISBN: 149393158X

Category: Gene expression

Page: 186

View: 127

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This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.
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Genetic Engineering of Crop Plants

1 ALL SORTS OF PLANT GENETIC MANIPULATION EDWARD C. COCKING
Plant Genetic Manipulation Group, University of Nottingham, Nottingham, UK
Introduction It is only about four years since the 41st Easter School was planned
 ...

Author: G. W. Lycett

Publisher: Elsevier

ISBN: 9781483100111

Category: Technology & Engineering

Page: 304

View: 540

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Genetic Engineering of Crop Plants is a proceeding of The 49th Nottingham Easter School in Agricultural Science, which was held at Sutton Bonington on April 17-21, 1989. This symposium discussed progress in the generation of crop species resistant to herbicides, viruses, and insects. The book discusses topics such as the genetic manipulation in plants; genetic engineering of crops for insect and herbicide resistance; the expression of heat shock gene in transgenic plants; and tuber-specific gene expression. The book also covers topics such as regulation of gene expression in transgenic tomato plants; the molecular biology of pea seed development; and the regulatory elements of maize storage protein genes. The text is recommended for experts in the field of botany, agriculture, and genetics who would like to know more about the improvement of crop plants through genetics.
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Genetic Modification of Plants

However, conventional breeding strategies depend on the availability of
significant genetic variation for a given trait within ... Genetic Modification of
Plants, Biotechnology in Agriculture and Forestry 64, DOI 10.1007/978-3-642-
02391-0_11, ...

Author: Frank Kempken

Publisher: Springer Science & Business Media

ISBN: 3642023916

Category: Science

Page: 675

View: 160

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Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book’s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.
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